Day 29 of 100: Bah.

Today’s experiment: some realtime on the primers that just came in the mail…



Well it doesn’t look terrible, except my No Template Control curve (bold green) is dangerously close to the rest of the curves. So I added some DNA loading dye to the well, ran it on a 2% agarose gel, and…


…it’s contaminated. The first band is from my No Template Control, and the second band is from another well in my realtime plate that I know will have product. The fact that these 2 are the same means that the primer is contaminated. Bahhhhh.

Yeah I’ll order new primer again tomorrow morning. Probably means another week or so until they arrive and I can try this again. Le sigh.


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