Today’s experiment: Western blot using that sweet loading control from last week.
Unfortunately, it looks like some protein was lost after I did the loading control and then “stripped” the membrane from last time. I think the protein I’m looking for is a bit sensitive like that.
I’ll be able to do another less-sensitive protein for tomorrow, but in order to do this one I’ll need to run another gel, and since I don’t have enough protein, I have to kill some mice first. Basically I’ll have to wait until the mice I’m using have babies again, who knows when that’ll be.