Day 72 of 100: Bummer.

Today’s experiment: Western blot using that sweet loading control from last week.

Result:

20150617_164514-1

Unfortunately, it looks like some protein was lost after I did the loading control and then “stripped” the membrane from last time. I think the protein I’m looking for is a bit sensitive like that.

I’ll be able to do another less-sensitive protein for tomorrow, but in order to do this one I’ll need to run another gel, and since I don’t have enough protein, I have to kill some mice first. Basically I’ll have to wait until the mice I’m using have babies again, who knows when that’ll be.

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s